rabbit anti human snail  (Cell Signaling Technology Inc)

Journal: Scientific Reports

Article Title: PAFAH1B3 is a KLF9 target gene that promotes proliferation and metastasis in pancreatic cancer

doi: 10.1038/s41598-024-59427-3

Figure Lengend Snippet: PAFAH1B3 affects the expression of EMT-related proteins in pancreatic cancer cells ( A ) SW1990 and MIA Paca-2 cells were transduced with lentivirus containing PAFAH1B3-Flag or NC, and the protein expression levels of PAFAH1B3, E-cadherin, N-cadherin, Vimentin, Snail1 and MMP2 were measured via Western blotting. ( B ) SW1990 and MIA Paca-2 cells were transduced with lentivirus containing sh-PAFAH1B3 or sh-NC, and the protein expression levels of PAFAH1B3, E-cadherin, N-cadherin, Vimentin, Snail1 and MMP2 were measured via Western blotting. β-Actin was used as an internal control. The data represent the average of three independent experiments. * p < 0.05; ** p < 0.01; *** p < 0.001.

Article Snippet: The following antibodies were used: rabbit anti-PAFAH1B3 polyclonal antibody (1:1000; Abcam, Cambridge, MA, USA), mouse anti-β-actin polyclonal antibody (1:1000; Boster Biological Technology, Ltd), rabbit anti-PCNA polyclonal antibody (1:1000; Abcam, Cambridge, MA, USA), rabbit anti-E-cadherin polyclonal antibody (1:1000; Boster Biological Technology, Ltd), rabbit anti-N-cadherin polyclonal antibody (1:1000; Boster Biological Technology, Ltd), rabbit anti-Snail1 polyclonal antibody (1:1000; Abcam, Cambridge, MA, USA), rabbit anti-MMP2 polyclonal antibody (1:1000; Abcam, Cambridge, MA, USA), rabbit anti-KLF9 polyclonal antibody (1:1000; Abcam, Cambridge, MA, USA), and mouse anti-vimentin polyclonal antibody (1:1000; Boster Biological Technology, Ltd.).

Techniques: Expressing, Transduction, Western Blot, Control